Amperometric lactate biosensor for flow injection analysis based on a screen-printed carbon electrode containing Meldola's Blue-Reinecke salt, coated with lactate dehydrogenase and NAD+
Piano, M., Serban, S., Pittson, R., Drago, G. and Hart, J. P. (2010) Amperometric lactate biosensor for flow injection analysis based on a screen-printed carbon electrode containing Meldola's Blue-Reinecke salt, coated with lactate dehydrogenase and NAD+. Talanta, 82 (1). pp. 34-37. ISSN 0039-9140 Available from: http://eprints.uwe.ac.uk/10748
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Publisher's URL: http://dx.doi.org/10.1016/j.talanta.2010.03.051
A biosensor for the measurement of lactate in serum has been developed, which is based on a screen-printed carbon electrode, modified with Meldola’s Blue-Reinecke Salt (MBRS-SPCE), coated with the enzyme lactate dehydrogenase NAD+ dependent (from Porcine heart), and NAD+. A cellulose acetate layer was deposited on the top of the device to act as a permselective membrane. The biosensor was incorporated into a commercially available, thin-layer, amperometric flow cell operated at a potential of only +0.05V vs. Ag/AgCl. The mobile phase consisted of 0.2M phosphate buffer pH 10 containing 0.1M potassium chloride solution; a flow rate of 0.8 ml min−1 was used throughout the investigation. The biosensor response was linear over the range 0.55–10mM lactate; the former represents the detection limit. The precision of the system was determined by carrying out 10 repeat injections of 10mM L(+)lactic acid standard; the calculated coefficient of variation was 4.28%. It was demonstrated that this biosensor system could be applied to the direct measurement of lactate in serum without pre-treatment; therefore, this would allow high throughput analysis, at low cost, for this clinically important analyte.