May, J. E., Xu, J., Morse, R., Avent, N. and Donaldson, C.
Toxicity testing: The search for an in vitro alternative to animal testing.
British Journal of Biomedical Science, 66 (3).
Publisher's URL: http://www.bjbs-online.org/article.asp?id=356
Prior to introduction to the clinic, pharmaceuticals must
undergo rigorous toxicity testing to ensure their safety.
Traditionally, this has been achieved using in vivo animal
models. However, besides ethical reasons, there is a
continual drive to reduce the number of animals used for
this purpose due to concerns such as the lack of
concordance seen between animal models and toxic effects
in humans. Adequate testing to ensure any toxic
metabolites are detected can be further complicated if the
agent is administered in a prodrug form, requiring a source
of cytochrome P450 enzymes for metabolism. A number of
sources of metabolic enzymes have been utilised in in vitro
models, including cell lines, primary human tissue and
liver extracts such as S9. This review examines current and
new in vitro models for toxicity testing, including a new
model developed within the authors’ laboratory utilising
HepG2 liver spheroids within a co-culture system to
examine the effects of chemotherapeutic agents on other
|Additional Information:||May, Jennifer E. and Xu, Jinsheng and Morse, Ruth and Avent, N.D. and Donaldson, Craig (2009) Toxicity testing: The search for an in vitro alternative to animal testing. British Journal of Biomedical Science, 66 (3). pp. 160-165. ISSN 0967-4845. This is the final draft, after peer-review, of a manuscript published in the British Journal of Biomedical Science. The definitive version, detailed above, is available online at www.rsmjournals.com.|
|Uncontrolled Keywords:||animal testing alternatives, Cytochrome P-450 enzyme system,
drug toxicity, prodrugs|
|Faculty/Department:||Faculty of Health and Applied Sciences > Department of Biological, Biomedical and Analytical Sciences|
Dr C. Donaldson
|Deposited On:||23 Sep 2010 12:33|
|Last Modified:||14 Aug 2013 00:13|
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