Presence of WT1 in nuclear messenger RNP particles in the human acute myeloid leukemia cell lines HL60 and K562

Morrison, A. A. and Ladomery, M. (2006) Presence of WT1 in nuclear messenger RNP particles in the human acute myeloid leukemia cell lines HL60 and K562. Cancer Letters, 244 (1). pp. 136-141. ISSN 0304-3835

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Publisher's URL: http://dx.doi.org/10.1016/j.canlet.2005.12.018

Abstract

The WT1 gene is a key player in acute myeloid leukaemia, in which it is frequently over-expressed. WT1 encodes a multifunctional zinc finger protein transcription factor, which also binds mRNA. Thus increasing evidence suggests that WT1 works both at the DNA and mRNA level, not only in the urogenital system but also in other contexts. Nuclear poly(A)+ mRNP particles were isolated by oligo(dT) chromatography from the human acute myeloid leukemia cell lines HL60 and K562, and analysed by Western blotting and 2D minigels. MALDI-TOF demonstrated the presence of hnRNP proteins, splice factors, and unexpectedly vimentin in the mRNP fraction. WT1 was also shown to be present in nuclear mRNP particles suggesting that in leukaemia, and by extension in all cancers in which it is involved, WT1 works both at the DNA and mRNA target level.

Item Type:Article
Uncontrolled Keywords:WT1, hnRNP H, mRNP proteomics, acute myeloid leukaemia, K562, HL60
Faculty/Department:Faculty of Health and Applied Sciences > Department of Biological, Biomedical and Analytical Sciences
ID Code:10899
Deposited By: A. Lawson
Deposited On:10 Aug 2010 13:47
Last Modified:12 Aug 2013 08:02

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