Magnetizable antibody-like proteins

Dehal, P. K., Livingston, C. F., Dunn, C. G., Buick, R., Luxton, R. W. and Pritchard, D. J. (2010) Magnetizable antibody-like proteins. Biotechnology Journal, 5 (6). pp. 596-604. ISSN 1860-6768

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Publisher's URL: http://dx.doi.org/10.1002/biot.200900273

Abstract

Use of paramagnetic particles to isolate molecules or cells from complex media is well established. Typically, particles are manufactured and coated with a biological molecule that confers specific biorecognition. Incubation of particles with sample and exposure to magnetic fields isolates the species of interest. We have designed, produced and assessed magnetized fusion proteins consisting of the antigen-binding portion of an antibody (single chain variable fraction; scFv) fused to the heavy chain of the iron-binding protein ferritin. The fusion protein subunits expressed in E. coli assemble to form a fusion protein consisting of a ferritin sphere with scFvs on the surface. The fusion proteins were chemically magnetized by introducing a paramagnetic iron core. The resultant fusion protein was shown to be magnetizable and capable of binding target antigens. These “organic” magnetizable particles possess a number of theoretical advantages over traditional inorganic particles.

Item Type:Article
Uncontrolled Keywords:analytical biotechnology, antibody, ferritin, paramagnetic, scFv
Faculty/Department:Faculty of Health and Applied Sciences > Department of Biological, Biomedical and Analytical Sciences
ID Code:12250
Deposited By: Professor R. Luxton
Deposited On:13 Jan 2011 09:10
Last Modified:06 Sep 2013 07:15

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