Magnetizable antibody-like proteins
Dehal, P. K., Livingston, C. F., Dunn, C. G., Buick, R., Luxton, R. W. and Pritchard, D. J. (2010) Magnetizable antibody-like proteins. Biotechnology Journal, 5 (6). pp. 596-604. ISSN 1860-6768 Available from: http://eprints.uwe.ac.uk/12250
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Publisher's URL: http://dx.doi.org/10.1002/biot.200900273
Use of paramagnetic particles to isolate molecules or cells from complex media is well established. Typically, particles are manufactured and coated with a biological molecule that confers specific biorecognition. Incubation of particles with sample and exposure to magnetic fields isolates the species of interest. We have designed, produced and assessed magnetized fusion proteins consisting of the antigen-binding portion of an antibody (single chain variable fraction; scFv) fused to the heavy chain of the iron-binding protein ferritin. The fusion protein subunits expressed in E. coli assemble to form a fusion protein consisting of a ferritin sphere with scFvs on the surface. The fusion proteins were chemically magnetized by introducing a paramagnetic iron core. The resultant fusion protein was shown to be magnetizable and capable of binding target antigens. These “organic” magnetizable particles possess a number of theoretical advantages over traditional inorganic particles.
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