Translationally controlled tumour protein (TCTP) is a novel glucose-regulated protein that is important for survival of pancreatic beta cells

Diraison, F., Hayward, K., Sanders, K. L., Brozzi, F., Lajus, S., Hancock, J. T., Fancis, J., Ainscow, E., Bommer, U., Molnar, E., Avent, N. and Varadi, A. (2010) Translationally controlled tumour protein (TCTP) is a novel glucose-regulated protein that is important for survival of pancreatic beta cells. Diabetologia, 54 (2). pp. 368-379. ISSN 0012-186X

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Publisher's URL: http://dx.doi.org/10.1007/s00125-010-1958-7

Abstract

Aims/hypothesis: This study used proteomics and biochemical approaches to identify novel glucose-regulated proteins and to unveil their role in pancreatic beta cell function. Translationally controlled tumour protein (TCTP) was identified to be one such protein, and further investigations into its function and regulation were carried out. Methods: Global protein profiling of beta cell homogenates following glucose stimulation was performed using twodimensional gel electrophoresis. Proteins were identified by mass spectroscopy analysis. Immunoblotting was used to investigate alterations in TCTP protein levels in response to glucose stimulation or cell stress induced by palmitate. To investigate the biological function of TCTP, immunolocalisation, gene knockdown and overexpression of Tctp (also known as Tpt1) were performed. Apoptosis was measured in Tctp knockdown or Tctp-overexpressing cells. Glucosestimulated insulin secretion was carried out in Tctp knockdown cells. Results: TCTP was identified as a novel glucose-regulated protein, the level of which is increased at stimulatory glucose concentration. Glucose also induced TCTP dephosphorylation and its partial translocation to the mitochondria and the nucleus. TCTP protein levels were downregulated in response to cell stress induced by palmitate or thapsigargin treatments. Gene knockdown by small interfering RNA led to increased apoptosis, whereas overproduction of TCTP prevented palmitate-induced cell death. Conclusions/interpretation: Regulation of TCTP protein levels by glucose is likely to be an important cyto-protective

Item Type:Article
Uncontrolled Keywords:apoptosis, fatty acid palmitate, insulin secretion, proteomics analysis, TCTP, translationally controlled tumour protein
Faculty/Department:Faculty of Health and Applied Sciences > Department of Biological, Biomedical and Analytical Sciences
ID Code:13737
Deposited By: C. Foyle
Deposited On:31 Jan 2011 12:56
Last Modified:12 Aug 2013 08:05

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