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Development of a fluorescent method for detecting the onset of coagulation in human plasma on microstructured lateral flow platforms

Dudek, Magdalena M.; Kent, Nigel J.; Killard, Anthony; Gu, Pan; Fan, Z. Hugh

Development of a fluorescent method for detecting the onset of coagulation in human plasma on microstructured lateral flow platforms Thumbnail


Authors

Magdalena M. Dudek

Nigel J. Kent

Pan Gu

Z. Hugh Fan



Abstract

Microfluidic devices and microsystems have been used to develop blood coagulation monitoring devices for point of care diagnostic use. However, many of them suffer from inherent variability and imprecision, partly due to the fact that they only detect changes in bulk clotting properties and do not reflect the microscopic nature of blood coagulation. This work demonstrates microstructured lateral flow platforms used in combination with fluorescently labelled fibrinogen to detect microscopic clot formation. Plasma samples applied to platforms modified with coagulation activation reagents and fluorescent fibrinogen produced changes in fluorescence intensity due to incorporation of the fluorophore into the forming microclots. It was found that the change in the distribution of the fluorescence within the sample over time was an excellent predictor of the onset of coagulation, which could be used to determine the clotting time. The impact of various assay parameters was optimised and the assay was shown to be capable of measuring the effect of heparin concentration on blood clotting time from 0 to 1.5 U mL-1. © 2011 The Royal Society of Chemistry.

Citation

Kent, N. J., Dudek, M. M., Gu, P., Fan, Z. H., & Killard, A. (2011). Development of a fluorescent method for detecting the onset of coagulation in human plasma on microstructured lateral flow platforms. Analyst, 136(9), 1816-1825. https://doi.org/10.1039/c0an00907e

Journal Article Type Article
Publication Date May 7, 2011
Deposit Date Mar 30, 2011
Publicly Available Date Feb 10, 2016
Journal Analyst
Print ISSN 0003-2654
Electronic ISSN 1364-5528
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 136
Issue 9
Pages 1816-1825
DOI https://doi.org/10.1039/c0an00907e
Keywords coagulation, assay device, point of care, activated partial thromboplastin time, diagnostics
Public URL https://uwe-repository.worktribe.com/output/965526
Publisher URL http://dx.doi.org/10.1039/c0an00907e

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