Development of a fluorescent assay for detecting the onset of coagulation in human plasma on microstructured, lateral flow platforms for point of care devices
Dudek, M., Kent, N., Gu, P., Fan, Z. H. and Killard, A.
Development of a fluorescent assay for detecting the onset of coagulation in human plasma on microstructured, lateral flow platforms for point of care devices.
The Analyst, 136 (9).
Available from: http://eprints.uwe.ac.uk/14118
Publisher's URL: http://dx.doi.org/10.1039/c0an00907e
Microfluidic devices and microsystems have been used to develop blood coagulation monitoring devices for point of care diagnostic use. However, many of them suffer from inherent variability and imprecision, partly due to the fact that they only detect changes in bulk clotting properties and do not reflect the microscopic nature of blood coagulation. This work demonstrates microstructured lateral flow platforms used in combination with fluorescently labelled fibrinogen to detect microscopic clot
formation. Plasma samples applied to platforms modified with coagulation activation reagents and fluorescent fibrinogen produced changes in fluorescence intensity due to incorporation of the fluorophore into the forming microclots. It was found that the change in the distribution of the fluorescence within the sample over time was an excellent predictor of the onset of coagulation, which could be used to determine the clotting time. The impact of various assay parameters was optimised and the assay was shown to be capable of measuring the effect of heparin concentration on blood clotting time from 0 to 1.5 U mL1.
|Uncontrolled Keywords:||coagulation, assay device, point of care, activated partial thromboplastin time, diagnostics|
Professor T. Killard
|Deposited On:||30 Mar 2011 11:05|
|Last Modified:||25 Mar 2017 16:59|
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