Suman, S., O'Reilly, E., Kelly, M., Morrin, A., Smyth, M. and Killard, A.
Chronocoulometric determination of urea in human serum using an inkjet printed biosensor.
Analytica Chimica Acta, 697 (1-2).
Available from: http://eprints.uwe.ac.uk/14425
Publisher's URL: http://dx.doi.org/10.1016/j.aca.2011.04.036
A biosensor for the determination of urea in human serum was fabricated using a combination of inkjet printed polyaniline nanoparticles and inkjet printed urease enzyme deposited sequentially onto screen-printed carbon paste electrodes. Chronocoulometry was used to measure the decomposition of urea via the doping of ammonium at the polyaniline-modified electrode surface at -0.3 V vs. Ag/AgCl. Ammonium could be measured in the range from 0.1 to 100 mM. Urea could be measured by the sensor in the range of 2 to 12 mM (r2=0.98). The enzyme biosensor was correlated against a spectrophotometric assay for urea in 15 normal human serum samples which yielded a correlation coefficient of 0.85. Bland-Altman plots showed that in the range of 5.8 to 6.6 mM urea, the developed sensor had an average positive experimental bias of 0.12 mM (<2% RSD) over the reference method.
|Uncontrolled Keywords:||biosensor, inkjet, polyaniline nanoparticles, urease, chronocoulometry|
Professor T. Killard
|Deposited On:||04 May 2011 15:01|
|Last Modified:||14 Aug 2013 08:50|
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