Chronocoulometric determination of urea in human serum using an inkjet printed biosensor

Suman, S., O'Reilly, E., Kelly, M., Morrin, A., Smyth, M. and Killard, A. (2011) Chronocoulometric determination of urea in human serum using an inkjet printed biosensor. Analytica Chimica Acta, 697 (1-2). pp. 98-102. ISSN 0003-2670

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Publisher's URL: http://dx.doi.org/10.1016/j.aca.2011.04.036

Abstract

A biosensor for the determination of urea in human serum was fabricated using a combination of inkjet printed polyaniline nanoparticles and inkjet printed urease enzyme deposited sequentially onto screen-printed carbon paste electrodes. Chronocoulometry was used to measure the decomposition of urea via the doping of ammonium at the polyaniline-modified electrode surface at -0.3 V vs. Ag/AgCl. Ammonium could be measured in the range from 0.1 to 100 mM. Urea could be measured by the sensor in the range of 2 to 12 mM (r2=0.98). The enzyme biosensor was correlated against a spectrophotometric assay for urea in 15 normal human serum samples which yielded a correlation coefficient of 0.85. Bland-Altman plots showed that in the range of 5.8 to 6.6 mM urea, the developed sensor had an average positive experimental bias of 0.12 mM (<2% RSD) over the reference method.

Item Type:Article
Uncontrolled Keywords:biosensor, inkjet, polyaniline nanoparticles, urease, chronocoulometry
ID Code:14425
Deposited By: Professor T. Killard
Deposited On:04 May 2011 15:01
Last Modified:14 Aug 2013 08:50

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