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Breed-associated variations in the sequence of the pig 3β- hydroxysteroid dehydrogenase gene

Cue, Rebecca A.; Nicolau-Solano, Silvia; McGivan, John D; Wood, Jeff D; Doran, O.

Authors

Rebecca A. Cue

Silvia Nicolau-Solano

John D McGivan

Jeff D Wood

Olena Doran Olena.Doran@uwe.ac.uk
College Dean of Research and Enterprise



Abstract

The entire sequence of the pig 3β-hydroxysteroid dehydrogenase (3β-HSD) gene has recently become known. This gene is deemed to be important in androstenone metabolism in pig liver, and its defective expression has been shown to be related to androstenone accumulation in adipose tissue and the development of boar taint. The aim of the present work was to do the following: 1) define the structure of the pig 3β-HSD gene and 2) compare 3β-HSD DNA sequences from pigs of different breeds, which vary in adipose tissue androstenone levels, with the purpose of identifying a polymorphism that might be responsible for differential 3β-HSD expression. The 5′ flanking and the coding region of 3β-HSD were cloned and sequenced by conventional techniques. The 3β-HSD coding regions were identical in pigs of different breeds and in animals with high and low androstenone levels. Significant sequence variations were found in the 5′ flanking region of the 3β-HSD gene, where differences in the number of TTAT repeats and 3 SNP were observed. The SNP were associated with the number of the TTAT repeats. These variations in the DNA sequence of the 3β-HSD gene were not associated with the androstenone level in s.c. adipose tissue but were breed-dependent. The results of this work might be used for detection of the presence of Meishan genes in Western pig breeds, especially if the phenotype is not clearly established. © 2007 American Society of Animal Science. All rights reserved.

Citation

Cue, R. A., Nicolau-Solano, S., McGivan, J. D., Wood, J. D., & Doran, O. (2007). Breed-associated variations in the sequence of the pig 3β- hydroxysteroid dehydrogenase gene. Journal of Animal Science, 85(3), 571-576. https://doi.org/10.2527/jas.2006-366

Journal Article Type Article
Publication Date Mar 1, 2007
Journal Journal of Animal Science
Print ISSN 0021-8812
Publisher Oxford University Press (OUP)
Peer Reviewed Peer Reviewed
Volume 85
Issue 3
Pages 571-576
DOI https://doi.org/10.2527/jas.2006-366
Keywords food quality, 3β-hydroxysteroid dehydrogenase, gene, pig, polymorphism
Public URL https://uwe-repository.worktribe.com/output/1032355
Publisher URL http://dx.doi.org/10.2527/jas.2006-366
Additional Information Corporate Creators : University of Bristol