Quantification of unfractionated heparin in human plasma and whole blood by means of novel fluorogenic anti-FXa assays

Killard, A. (2011) Quantification of unfractionated heparin in human plasma and whole blood by means of novel fluorogenic anti-FXa assays. Journal of Pharmaceutical and Biomedical Analysis, 56 (5). pp. 992-997. ISSN 0731-7085

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Publisher's URL: http://dx.doi.org/10.1016/j.jpba.2011.07.025

Abstract

Novel and sensitive plate-based fluorogenic anti-factor Xa (FXa) assays were investigated to quantify unfractionated heparin (UFH) in human plasma and whole blood within the therapeutic ranges of 0–1.6 U/mL and 0–0.8 U/mL, respectively. Two fluorogenic anti-FXa assay methods were defined for low (0–0.6 U/mL) and high (0.6–1.2 U/mL) pharmacologically relevant UFH concentration ranges in pooled human plasma. In both cases significant differences were observed at intervals of 0.2 U/mL (P < 0.05). The semi-logarithmic plots of the calibration curves in the low and high UFH range were both fitted to linear regressions with correlation coefficients of 0.96 and >0.99, respectively. The assay was also optimized for whole blood which was capable of differentiating UFH concentrations at intervals of 0.2 U/mL (P < 0.05) in the range of 0–0.4 U/mL. The statistically different results were fitted to a linear regression with a correlation coefficient of >0.99. The results obtained in this study could assist diagnostic laboratories towards improved monitoring of UFH therapy.

Item Type:Article
Uncontrolled Keywords:fluorogenic anti-FXa assay, whole blood, plasma, UFH, monitoring
ID Code:15653
Deposited By: Professor T. Killard
Deposited On:13 Sep 2011 11:55
Last Modified:12 Aug 2013 08:06

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