Novel electrode reactions of diazepam, flunitrazepam and lorazepam and their exploitation in a new redox mode LC-DED assay for serum

Honeychurch, K. C., Chong, A., Elamin, K. and Hart, J. P. (2012) Novel electrode reactions of diazepam, flunitrazepam and lorazepam and their exploitation in a new redox mode LC-DED assay for serum. Analytical Methods, 4 (1). pp. 132-140. ISSN 1759-9660

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Publisher's URL: http://dx.doi.org/10.1039/C1AY05419H

Abstract

Previously unreported voltammetric redox behaviour was identified for flunitrazepam, lorazepam and diazepam at a glassy carbon electrode. This was successfully exploited for their determination in serum by high performance liquid chromatography dual electrode detection in the redox mode (LC-DED). Initial studies were performed to optimise the chromatographic conditions and these were found to be 50% acetone, 50% 100 mM pH 2 phosphate buffer at a flow rate of 0.8 ml min−1, employing a Hypersil C18, 5 μm, 250 mm × 4.6 mm column held at 40 °C. Cyclic voltammetric studies were made to ascertain the redox behaviour of these benzodiazepines over the pH range 2–10. Hydrodynamic voltammetry was used to optimise the applied potential at the amperometric generator and detector cells; these were identified to be −2.45 V (vs. stainless steel) and +1.0 V. (vs. Ag/AgCl).

Item Type:Article
Uncontrolled Keywords:liquid chromatography, electrochemical detection, diazepam, flunitrazepam and lorazepam
Faculty/Department:Faculty of Health and Applied Sciences > Department of Biological, Biomedical and Analytical Sciences
ID Code:16504
Deposited By: Dr K. Honeychurch
Deposited On:08 Feb 2012 09:56
Last Modified:20 Aug 2013 14:27

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