Dr Kevin Honeychurch Kevin.Honeychurch@uwe.ac.uk
Senior Lecturer in Forensic Science
Novel electrode reactions of diazepam, flunitrazepam and lorazepam and their exploitation in a new redox mode LC-DED assay for serum
Honeychurch, Kevin C.; Chong, Ai Teng; Elamin, Khalil; Hart, John P.
Authors
Ai Teng Chong
Khalil Elamin
John Hart John.Hart@uwe.ac.uk
Professor
Abstract
Previously unreported voltammetric redox behaviour was identified for flunitrazepam, lorazepam and diazepam at a glassy carbon electrode. This was successfully exploited for their determination in serum by high performance liquid chromatography dual electrode detection in the redox mode (LC-DED). Initial studies were performed to optimise the chromatographic conditions and these were found to be 50% acetone, 50% 100 mM pH 2 phosphate buffer at a flow rate of 0.8 ml min -1, employing a Hypersil C 18, 5 μm, 250 mm × 4.6 mm column held at 40 °C. Cyclic voltammetric studies were made to ascertain the redox behaviour of these benzodiazepines over the pH range 2-10. Hydrodynamic voltammetry was used to optimise the applied potential at the amperometric generator and detector cells; these were identified to be -2.45 V (vs. stainless steel) and +1.0 V. (vs. Ag/AgCl). © 2012 The Royal Society of Chemistry.
Citation
Honeychurch, K. C., Chong, A. T., Elamin, K., & Hart, J. P. (2012). Novel electrode reactions of diazepam, flunitrazepam and lorazepam and their exploitation in a new redox mode LC-DED assay for serum. Analytical Methods, 4(1), 132-140. https://doi.org/10.1039/c1ay05419h
Journal Article Type | Article |
---|---|
Publication Date | Jan 1, 2012 |
Journal | Analytical Methods |
Print ISSN | 1759-9660 |
Electronic ISSN | 1759-9679 |
Publisher | Royal Society of Chemistry |
Peer Reviewed | Peer Reviewed |
Volume | 4 |
Issue | 1 |
Pages | 132-140 |
DOI | https://doi.org/10.1039/c1ay05419h |
Keywords | liquid chromatography, electrochemical detection, diazepam, flunitrazepam and lorazepam |
Public URL | https://uwe-repository.worktribe.com/output/953521 |
Publisher URL | http://dx.doi.org/10.1039/C1AY05419H |
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