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Novel electrode reactions of diazepam, flunitrazepam and lorazepam and their exploitation in a new redox mode LC-DED assay for serum

Honeychurch, Kevin C.; Chong, Ai Teng; Elamin, Khalil; Hart, John P.

Authors

Ai Teng Chong

Khalil Elamin



Abstract

Previously unreported voltammetric redox behaviour was identified for flunitrazepam, lorazepam and diazepam at a glassy carbon electrode. This was successfully exploited for their determination in serum by high performance liquid chromatography dual electrode detection in the redox mode (LC-DED). Initial studies were performed to optimise the chromatographic conditions and these were found to be 50% acetone, 50% 100 mM pH 2 phosphate buffer at a flow rate of 0.8 ml min -1, employing a Hypersil C 18, 5 μm, 250 mm × 4.6 mm column held at 40 °C. Cyclic voltammetric studies were made to ascertain the redox behaviour of these benzodiazepines over the pH range 2-10. Hydrodynamic voltammetry was used to optimise the applied potential at the amperometric generator and detector cells; these were identified to be -2.45 V (vs. stainless steel) and +1.0 V. (vs. Ag/AgCl). © 2012 The Royal Society of Chemistry.

Citation

Honeychurch, K. C., Chong, A. T., Elamin, K., & Hart, J. P. (2012). Novel electrode reactions of diazepam, flunitrazepam and lorazepam and their exploitation in a new redox mode LC-DED assay for serum. Analytical Methods, 4(1), 132-140. https://doi.org/10.1039/c1ay05419h

Journal Article Type Article
Publication Date Jan 1, 2012
Journal Analytical Methods
Print ISSN 1759-9660
Electronic ISSN 1759-9679
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 4
Issue 1
Pages 132-140
DOI https://doi.org/10.1039/c1ay05419h
Keywords liquid chromatography, electrochemical detection, diazepam, flunitrazepam and lorazepam
Public URL https://uwe-repository.worktribe.com/output/953521
Publisher URL http://dx.doi.org/10.1039/C1AY05419H