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Dietary glycated protein modulates the colonic microbiota towards a more detrimental composition in ulcerative colitis patients and non-ulcerative colitis subjects

Mills, D.J.S; Tuohy, K.M; Crabbe, M.J.C; Gibson, G.R; Ames, Jennifer; Booth, J.; Buck, M.

Authors

D.J.S Mills

K.M Tuohy

M.J.C Crabbe

G.R Gibson

Jennifer Ames

J. Booth

M. Buck



Abstract

Aim: To investigate the effect of native, heated and glycated bovine serum albumin (BSA) on the ulcerative colitis (UC) and non-UC colonic microbiota in vitro. Methods and Results: Continuous flow culture (CFC) models of the human colonic microbiota inoculated with faeces from UC and non-UC volunteers were maintained on BSA as growth substrate. Changes in bacterial populations and short-chain fatty acids were determined. UC and non-UC microbiota differed significantly in microbial populations, with elevated numbers of sulfate-reducing bacteria (SRB) and clostridia in the microbiota from UC patients. Compared with native BSA, glycated BSA modulated the gut microbiota of UC patients in vitro towards a more detrimental community structure with significant increases in putatively harmful bacteria (clostridia, bacteroides and SRB; P < 0.009) and decreases in dominant and putatively beneficial bacterial groups (eubacteria and bifidobacteria; P < 0.0004). The levels of beneficial short-chain fatty acids were significantly decreased by heated or glycated BSA, but were increased significantly by native BSA. Conclusion: The UC colonic microbiota maintained in CFC was significantly modified by glycated BSA. Significance and Impact of the Study: Results suggest that dietary glycated protein may impact upon the composition and activity of the colonic microbiota, an important environmental variable in UC. © 2008 The Authors.

Citation

Mills, D., Tuohy, K., Booth, J., Buck, M., Crabbe, M., Gibson, G., & Ames, J. (2008). Dietary glycated protein modulates the colonic microbiota towards a more detrimental composition in ulcerative colitis patients and non-ulcerative colitis subjects. Journal of Applied Microbiology, 105(3), 706-714. https://doi.org/10.1111/j.1365-2672.2008.03783.x

Journal Article Type Article
Publication Date Sep 1, 2008
Journal Journal of Applied Microbiology
Print ISSN 1364-5072
Electronic ISSN 1365-2672
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 105
Issue 3
Pages 706-714
DOI https://doi.org/10.1111/j.1365-2672.2008.03783.x
Keywords colonic microbiota, continuous flow culture,glycated protein, short-chain fatty acids,sulfate-reducing bacteria, lcerative colitis
Public URL https://uwe-repository.worktribe.com/output/1009785
Publisher URL http://dx.doi.org/10.1111/j.1365-2672.2008.03783.x


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