The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees

Quainoo, A. K., Wetten, A. C. and Allainguillaume, J. (2008) The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees. Journal of Virological Methods, 149 (1). pp. 91-96. ISSN 0166-0934

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Publisher's URL: http://dx.doi.org/10.1016/j.jviromet.2008.01.007

Abstract

Investigations were undertaken on the use of somatic embryogenesis to generate cocoa swollen shoot virus (CSSV) disease free clonal propagules from infected trees. Polymerase chain reaction (PCR) capillary electrophoresis revealed the presence of CSSV in all the callus tissues induced from the CSSV-infected Amelonado cocoa trees (T1, T2 and T4). The virus was transmitted to primary somatic embryos induced from the infected callus tissues at the rate of 10 (19%), 18 (14%) and 16 (15%) for T1, T2 and T4, respectively. Virus free primary somatic embryos from the infected callus tissues converted into plantlets tested CSSV negative by PCR/capillary electrophoresis 2 years after weaning. Secondary somatic embryos induced from the CSSV-infected primary somatic embryos revealed the presence of viral fragments at the rate of 4 (4%) and 9 (9%) for T2 and T4, respectively. Real-time PCR revealed 23 of the 24 secondary somatic embryos contained no detectable virus. Based on these findings, it isproposed that progressive elimination of the CSSV in infected cocoa trees occurred from primary embryogenesis to secondary embryogenesis.

Item Type:Article
Uncontrolled Keywords:cocoa swollen shoot virus (CSSV), primary embryogenesis, secondary embryogenesis, somatic embryos, PCR/capillary electrophoresis, real-time polymerase chain reaction
Faculty/Department:Faculty of Health and Applied Sciences > Department of Biological, Biomedical and Analytical Sciences
ID Code:17340
Deposited By: L. Armstrong-James
Deposited On:21 Sep 2012 13:47
Last Modified:31 Jul 2013 14:27

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