Skip to main content

Research Repository

Advanced Search

Evaluation of a silver-based electrocatalyst for the determination of hydrogen peroxide formed via enzymatic oxidation

Smyth, Malcolm R.; Gonzalez-Macia, Laura; Killard, Anthony

Authors

Malcolm R. Smyth

Laura Gonzalez-Macia



Abstract

Silver paste electrodes modified with lyotropic phases formed from dodecyl benzenesulphonic acid and KCl were used as the reductant in the determination of the hydrogen peroxide released from the enzymatic reaction of glucose oxidase with glucose and oxygen. The response of the modified electrode to hydrogen peroxide reduction (-0.1 V vs. Ag/AgCl) was shown to suffer from interference resulting from co-localization of enzyme and substrate at the electrode surface. This interference was eradicated by the introduction of a perm-selective membrane in the form of cellulose acetate. This further facilitated immobilization of the enzyme while allowing diffusion of the generated peroxide to the electrode. The resulting configuration was shown to be capable of the analytical determination of glucose. © 2012 Elsevier B.V.

Citation

Smyth, M. R., Gonzalez-Macia, L., & Killard, A. (2012). Evaluation of a silver-based electrocatalyst for the determination of hydrogen peroxide formed via enzymatic oxidation. Talanta, 99, 989-996. https://doi.org/10.1016/j.talanta.2012.07.082

Journal Article Type Article
Publication Date Sep 15, 2012
Journal Talanta
Print ISSN 0039-9140
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 99
Pages 989-996
DOI https://doi.org/10.1016/j.talanta.2012.07.082
Keywords silver, screen-printed electrode, hydrogen peroxide, dodecylbenzene sulphonic acid, glucose, glucose oxidase
Public URL https://uwe-repository.worktribe.com/output/953122
Publisher URL http://dx.doi.org/10.1016/j.talanta.2012.07.082