Malcolm R. Smyth
Evaluation of a silver-based electrocatalyst for the determination of hydrogen peroxide formed via enzymatic oxidation
Smyth, Malcolm R.; Gonzalez-Macia, Laura; Killard, Anthony
Authors
Laura Gonzalez-Macia
Prof. Tony Killard Tony.Killard@uwe.ac.uk
Research Professor in Biomedical Sciences
Abstract
Silver paste electrodes modified with lyotropic phases formed from dodecyl benzenesulphonic acid and KCl were used as the reductant in the determination of the hydrogen peroxide released from the enzymatic reaction of glucose oxidase with glucose and oxygen. The response of the modified electrode to hydrogen peroxide reduction (-0.1 V vs. Ag/AgCl) was shown to suffer from interference resulting from co-localization of enzyme and substrate at the electrode surface. This interference was eradicated by the introduction of a perm-selective membrane in the form of cellulose acetate. This further facilitated immobilization of the enzyme while allowing diffusion of the generated peroxide to the electrode. The resulting configuration was shown to be capable of the analytical determination of glucose. © 2012 Elsevier B.V.
Citation
Smyth, M. R., Gonzalez-Macia, L., & Killard, A. (2012). Evaluation of a silver-based electrocatalyst for the determination of hydrogen peroxide formed via enzymatic oxidation. Talanta, 99, 989-996. https://doi.org/10.1016/j.talanta.2012.07.082
Journal Article Type | Article |
---|---|
Publication Date | Sep 15, 2012 |
Journal | Talanta |
Print ISSN | 0039-9140 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 99 |
Pages | 989-996 |
DOI | https://doi.org/10.1016/j.talanta.2012.07.082 |
Keywords | silver, screen-printed electrode, hydrogen peroxide, dodecylbenzene sulphonic acid, glucose, glucose oxidase |
Public URL | https://uwe-repository.worktribe.com/output/953122 |
Publisher URL | http://dx.doi.org/10.1016/j.talanta.2012.07.082 |
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