Electrochemical detection of extracellular hydrogen peroxide in Arabidopsis thaliana: A real-time marker of oxidative stress

Gonzalez-Sanchez, M. I., Gonzalez-Macia, L., Hancock, J. T. and Killard, A. (2013) Electrochemical detection of extracellular hydrogen peroxide in Arabidopsis thaliana: A real-time marker of oxidative stress. Plant, Cell and Environment, 36 (4). pp. 869-878. ISSN 0140-7791

Full text not available from this repository

Publisher's URL: http://dx.doi.org/10.1111/pce.12023

Abstract

An electrochemical approach to directly measure the dynamic process of H2O2 release from cultures of Arabidopsis thaliana cells is reported. This approach is based on H2O2 oxidation on a Pt electrode in conjunction with continuous measurement of sample pH. For [H2O2] < 1 mM, calibration plots were linear and the amperometric response of the electrode was dependent on the pH. At higher concentrations ([H2O2] > 1 mM), the amperometric response can be described by Michaelian-type kinetics and a mathematical expression relating current intensity and pH was obtained to quantitatively determine H2O2 concentration. At pH 5.5, the detection limit of the sensor was 3.1 M (S/N=3), with a response sensitivity of 0.16 AM-1cm-2 and reproducibility was within 6.1% in the range 1-5 x 10-3 M (n=5). Cell suspensions under normal physiological conditions had a pH between 5.5-5.7 and H2O2 concentrations in the range 7.0-20.5 M (n=5). The addition of exogenous H2O2, as well as other potential stress stimuli was made to the cells and the change in H2O2 concentration was monitored. This real-time quantitative H2O2 analysis is a potential marker for the evaluation of oxidative stress in plant cell cultures.

Item Type:Article
Uncontrolled Keywords:hydrogen peroxide, plant cells, oxidative stress
Faculty/Department:Faculty of Health and Applied Sciences > Department of Biological, Biomedical and Analytical Sciences
ID Code:17679
Deposited By: Professor T. Killard
Deposited On:09 Nov 2012 10:10
Last Modified:31 Jan 2014 18:59

Request a change to this item

Document Downloads

Total Document Downloads

More statistics for this item...
Copyright 2013 © UWE better together