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Expression of phosphatidylserine (PS) on wild-type and Gerbich variant erythrocytes following glycophorin-C (GPC) ligation

Head, David J.; Lee, Zoe E.; Poole, Joyce; Avent, Neil D.

Authors

David J. Head

Zoe E. Lee

Joyce Poole

Neil D. Avent



Abstract

Glycophorin-C (GPC) is a 40 kDa glycoprotein expressed on erythrocytes and is a receptor for the malarial parasite Plasmodium falciparum to invade these cells. A link between GPC binding (ligation) and phosphatidylserine (PS) expression on erythrocytes has been suggested by its appearance on P. falsiparum-infected erythrocytes. Phosphatidylserine expression has also been shown to be a marker of cellular death in a number of biological pathways including some in erythrocytes. Using Annexin V binding, we demonstrated that ligation of GPC with mouse mAb (BRIC-10) induced PS expression on normal erythrocytes. Phosphatidylserine exposure was prevented following tryptic digestion of intact erythrocytes. In addition, GPC variant phenotypes Yus (Δ exon 2) and Gerbich (Δ exon 3), which express a truncated extracellular domain, did not express PS following BRIC-10 binding, whereas PS was exposed on Lsa erythrocytes (duplication of exon 3). GPC ligation was also shown to result in a concomitant loss of erythrocyte viability in wild-type erythrocytes after 24 h in vitro. These results identify a potential pathway linking GPC to PS exposure on erythrocytes that may have a role in regulating red cell turnover. Further characterization of this pathway may also identify new targets for the treatment of P. falciparum malaria. © 2005 Blackwell Publishing Ltd.

Citation

Head, D. J., Lee, Z. E., Poole, J., & Avent, N. D. (2005). Expression of phosphatidylserine (PS) on wild-type and Gerbich variant erythrocytes following glycophorin-C (GPC) ligation. British Journal of Haematology, 129(1), 130-137. https://doi.org/10.1111/j.1365-2141.2005.05407.x

Journal Article Type Article
Publication Date Apr 1, 2005
Journal British Journal of Haematology
Print ISSN 0007-1048
Publisher Wiley
Peer Reviewed Not Peer Reviewed
Volume 129
Issue 1
Pages 130-137
DOI https://doi.org/10.1111/j.1365-2141.2005.05407.x
Keywords erythrocyte, glycophorin-C, phosphatidylserine, Gerbich variants
Public URL https://uwe-repository.worktribe.com/output/1051994
Publisher URL http://dx.doi.org/10.1111/j.1365-2141.2005.05407.x


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