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Chromosome translocations and covert leukemic clones are generated during normal fetal development

Mori, H., Colman, S., Xiao, Z., Ford, A., Healy, L., Donaldson, C., Hows, J. M., Navarrete, C. and Greaves, M. (2002) Chromosome translocations and covert leukemic clones are generated during normal fetal development. PNAS, 99 (12). pp. 8242-8247. ISSN 0027-8424

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Publisher's URL: http://dx.doi.org/10.1073/pnas.112218799

Abstract

Studies on monozygotic twins with concordant leukemia and retrospective scrutiny of neonatal blood spots of patients with leukemia indicate that chromosomal translocations characteristic of pediatric leukemia often arise prenatally, probably as initiating events. The modest concordance rate for leukemia in identical twins (≈5%), protracted latency, and transgenic modeling all suggest that additional postnatal exposure and/or genetic events are required for clinically overt leukemia development. This notion leads to the prediction that chromosome translocations, functional fusion genes, and preleukemic clones should be present in the blood of healthy newborns at a rate that is significantly greater than the cumulative risk of the corresponding leukemia. Using parallel reverse transcriptase–PCR and real-time PCR (Taqman) screening, we find that the common leukemia fusion genes, TEL-AML1 or AML1-ETO, are present in cord bloods at a frequency that is 100-fold greater than the risk of the corresponding leukemia. Single-cell analysis by cell enrichment and immunophenotype/fluorescence in situ hybridization multicolor staining confirmed the presence of translocations in restricted cell types corresponding to the B lymphoid or myeloid lineage of the leukemias that normally harbor these fusion genes. The frequency of positive cells (10−4 to 10−3) indicates substantial clonal expansion of a progenitor population. These data have significant implications for the pathogenesis, natural history, and etiology of childhood leukemia.

Item Type:Article
Additional Information:This paper provided molecular biologic evidence indicating that most cases of acute leukaemia in infants are initiated by chromosomal and genetic alterations prenatally, although leukaemia initiation by chromosomal changes was far more common than the rarity of the disease would suggest and giving rise to the multi-hit theory.
Uncontrolled Keywords:chromosome translocations, covert leukemic clones, normal fetal development
Faculty/Department:Faculty of Environment and Technology
ID Code:5808
Deposited By: R. Upload account
Deposited On:22 Jan 2010 15:10
Last Modified:22 Apr 2015 07:36

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