Towards the development of molecularly imprinted polymer based screen-printed sensors for metabolites of PAHs
Kirsch, N., Hart, J. P., Bird, D. J., Luxton, R. W. and McCalley, D. V. (2001) Towards the development of molecularly imprinted polymer based screen-printed sensors for metabolites of PAHs. The Analyst, 126 (11). pp. 1936-1941. ISSN 0003-2654
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Publisher's URL: http://dx.doi.org/10.1039/b108008n
This paper describes the fabrication of a sensor for 1-hydroxypyrene (1-OHP) based on a screen-printed carbon electrode (SPCE) modified with a molecularly imprinted polymer (MIP); 1-OHP was chosen as a model metabolite of polyaromatic hydrocarbons (PAHs). It was shown that 1-OHP could be readily oxidised at a plain SPCE and the electrochemical mechanism was found to involve an ECE (electron transfer–chemical reaction–electron transfer) process. The MIP for 1-OHP was prepared using only divinylbenzene (DVB) and styrene as monomers and the binding was only based on hydrophobic interactions. Batch binding studies revealed that optimum uptake of 1-OHP by the MIP occurred from solutions containing 35% water in methanol. Selectivity of the binding sites in the MIP was examined by performing uptake studies in the same solution containing either phenol or 1-naphthol; the specific binding of 1-OHP was twenty times greater than the former and five times greater than the latter. Preliminary calibration studies were performed with the MIP-SPCE using a two-step approach; accumulation was carried out in 35% water in methanol followed by measurement in 50% methanol–0.025 mol dm−3 phosphate buffer pH 12. This two-step non-competitive affinity assay gave encouraging results and indicated potential for use in pollution studies.
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