Towards the development of molecularly imprinted polymer based screen-printed sensors for metabolites of PAHs
Kirsch, N. , Hart, J. P. , Bird, D. J. , Luxton, R. W. and McCalley, D. V. (2001) Towards the development of molecularly imprinted polymer based screen-printed sensors for metabolites of PAHs. The Analyst, 126 (11). pp. 1936-1941. ISSN 0003-2654
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Publisher's URL: http://dx.doi.org/10.1039/b108008n
This paper describes the fabrication of a sensor for 1-hydroxypyrene (1-OHP) based on a screen-printed carbon electrode (SPCE) modified with a molecularly imprinted polymer (MIP); 1-OHP was chosen as a model metabolite of polyaromatic hydrocarbons (PAHs). It was shown that 1-OHP could be readily oxidised at a plain SPCE and the electrochemical mechanism was found to involve an ECE (electron transfer–chemical reaction–electron transfer) process. The MIP for 1-OHP was prepared using only divinylbenzene (DVB) and styrene as monomers and the binding was only based on hydrophobic interactions. Batch binding studies revealed that optimum uptake of 1-OHP by the MIP occurred from solutions containing 35% water in methanol. Selectivity of the binding sites in the MIP was examined by performing uptake studies in the same solution containing either phenol or 1-naphthol; the specific binding of 1-OHP was twenty times greater than the former and five times greater than the latter. Preliminary calibration studies were performed with the MIP-SPCE using a two-step approach; accumulation was carried out in 35% water in methanol followed by measurement in 50% methanol–0.025 mol dm−3 phosphate buffer pH 12. This two-step non-competitive affinity assay gave encouraging results and indicated potential for use in pollution studies.
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