Efficient GFP expression in the mushrooms and requires introns

Burns, C. , Gregory, K. , Kirby, M. , Cheung, M.-K. , Riquelme, M. , Elliott, T. , Challen, M. , Bailey, A. and Foster, G. (2005) Efficient GFP expression in the mushrooms and requires introns. Fungal Genetics and Biology, 42 (3). pp. 191-199. ISSN 1087-1845

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Publisher's URL: http://dx.doi.org/10.1016/j.fgb.2004.11.005

Abstract

We have developed a “Molecular Toolkit” comprising interchangeable promoters and marker genes to facilitate transformation of homobasidiomycete mushrooms. We describe the evaluation of a range of promoters in the homobasidiomycetes Agaricus bisporus and Coprinus cinereus using green fluorescent protein (GFP) as a reporter gene; the C. cinereus trp1 promoter and A. bisporus trp2 and gpdII promoters proving successful in driving expression in C. cinereus, with the gpdII promoter also functioning in A. bisporus. Our investigations demonstrate that a prerequisite for GFP expression in C. cinereus and A. bisporus is the presence of an intron. This is the first reported expression of GFP in either C. cinereus or A. bisporus.

Item Type:	Article
Faculty/Department:	~Pre-2010 Faculty Structure > Health and Life Sciences > School of Life Sciences ~Pre-2012 Faculty Structure > Faculty of Health and Life Sciences Faculty of Health and Life Sciences
ID Code:	7064
Deposited By:	H. Hammond
Deposited On:	26 Jul 2010 15:40
Last Modified:	22 Nov 2012 15:36