Stephen Smith
A comparative study of the analysis of human urine headspace using gas chromatography-mass spectrometry
Smith, Stephen; Burden, H.; de Lacy Costello, Ben; Persad, R.; Ratcliffe, Norman M.; Whittington, K.; Probert, C. S.
Authors
H. Burden
Benjamin De Lacy Costello Ben.DeLacyCostello@uwe.ac.uk
Associate Professor in Diagnostics and Bio-Sensing Technology
R. Persad
Norman Ratcliffe Norman.Ratcliffe@uwe.ac.uk
Professor in Materials & Sensors Science
K. Whittington
C. S. Probert
Abstract
First-void urine samples were obtained from 24 elderly, asymptomatic men (median age 62.9 years). The headspace above pH adjusted urine samples were extracted using a carboxen/polydimethylsiloxane solid phase micro-extraction fibre and the volatile organic compounds analysed by gas chromatography/mass spectrometry. A total of 147 compounds were identified in the headspace of urine. The acidified samples recorded a total of 92 compounds, 27 of which were ubiquitous, basified samples 70 compounds, with 12 ubiquitous and unmodified pH samples 49, with 6 ubiquitous. Five compounds were ubiquitous irrespective of pH: acetone, methylene chloride, 4-heptanone, 2-pentanone and 2-butanone. A comparative analysis of unfrozen and frozen-thawed urine (stored at room temperature for 0, 1 and 8 h) showed that samples retained the same number of compounds although variations in the peak areas for some compounds were observed. © 2008 IOP Publishing Ltd.
Citation
Smith, S., Burden, H., Persad, R., Whittington, K., de Lacy Costello, B., Ratcliffe, N. M., & Probert, C. S. (2008). A comparative study of the analysis of human urine headspace using gas chromatography-mass spectrometry. Journal of Breath Research, 2(3), 037022. https://doi.org/10.1088/1752-7155/2/3/037022
Journal Article Type | Article |
---|---|
Publication Date | Sep 1, 2008 |
Journal | Journal of Breath Research |
Print ISSN | 1752-7155 |
Electronic ISSN | 1752-7163 |
Publisher | IOP Publishing |
Peer Reviewed | Peer Reviewed |
Volume | 2 |
Issue | 3 |
Pages | 037022 |
DOI | https://doi.org/10.1088/1752-7155/2/3/037022 |
Public URL | https://uwe-repository.worktribe.com/output/1022081 |
Publisher URL | http://dx.doi.org/10.1088/1752-7155/2/3/037022 |