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A comparative study of the analysis of human urine headspace using gas chromatography-mass spectrometry

Smith, Stephen; Burden, H.; de Lacy Costello, Ben; Persad, R.; Ratcliffe, Norman M.; Whittington, K.; Probert, C. S.

Authors

Stephen Smith

H. Burden

R. Persad

K. Whittington

C. S. Probert



Abstract

First-void urine samples were obtained from 24 elderly, asymptomatic men (median age 62.9 years). The headspace above pH adjusted urine samples were extracted using a carboxen/polydimethylsiloxane solid phase micro-extraction fibre and the volatile organic compounds analysed by gas chromatography/mass spectrometry. A total of 147 compounds were identified in the headspace of urine. The acidified samples recorded a total of 92 compounds, 27 of which were ubiquitous, basified samples 70 compounds, with 12 ubiquitous and unmodified pH samples 49, with 6 ubiquitous. Five compounds were ubiquitous irrespective of pH: acetone, methylene chloride, 4-heptanone, 2-pentanone and 2-butanone. A comparative analysis of unfrozen and frozen-thawed urine (stored at room temperature for 0, 1 and 8 h) showed that samples retained the same number of compounds although variations in the peak areas for some compounds were observed. © 2008 IOP Publishing Ltd.

Citation

Smith, S., Burden, H., Persad, R., Whittington, K., de Lacy Costello, B., Ratcliffe, N. M., & Probert, C. S. (2008). A comparative study of the analysis of human urine headspace using gas chromatography-mass spectrometry. Journal of Breath Research, 2(3), 037022. https://doi.org/10.1088/1752-7155/2/3/037022

Journal Article Type Article
Publication Date Sep 1, 2008
Journal Journal of Breath Research
Print ISSN 1752-7155
Electronic ISSN 1752-7163
Publisher IOP Publishing
Peer Reviewed Peer Reviewed
Volume 2
Issue 3
Pages 037022
DOI https://doi.org/10.1088/1752-7155/2/3/037022
Public URL https://uwe-repository.worktribe.com/output/1022081
Publisher URL http://dx.doi.org/10.1088/1752-7155/2/3/037022