Fungal elicitor Pep-25 increases cytosolic calcium ions, H2O2 production and activates the octadecanoid pathway in Arabidopsis thaliana

Hu, X. , Neill, S. , Yang, Y. and Cai, W. (2009) Fungal elicitor Pep-25 increases cytosolic calcium ions, H2O2 production and activates the octadecanoid pathway in Arabidopsis thaliana. Planta, 229 (6). pp. 1201-1208. ISSN 0032-0935

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Publisher's URL: http://dx.doi.org/10.1007/s00425-009-0909-3

Abstract

Transgenic Arabidopsis thaliana plants expressing the protein aequorin were used to investigate the transient change in cytosolic calcium ions caused by stimulation through the fungal elicitor Pep-25. Our results show that the elicitor Pep-25 derived from Phytophthora sojae can induce H2O2 production and an increase in cytosolic calcium ions. The transcription of LOX, OPR3, PED, AOS, AOC genes and the protein accumulation of AOS were induced by Pep-25 treatment. Pep-25 also induced an accumulation of jasmonic acid (JA). Blocking the production of H2O2 and the increase of cytosolic calcium ions both suppressed the transcription of LOX, OPR3, PED, AOS, AOC genes, the accumulation of AOS, and the accumulation of JA. These results indicated that the production of H2O2 derived from the plasma-membrane NADPH oxidase and the subsequently increase of cytosolic calcium ions are both required for the activation of the octadecanoid pathway by Pep-25 treatment in A. thaliana.

Item Type:	Article
Uncontrolled Keywords:	Arabidopsis, aequorin, cytosolic calcium ions, hydrogen peroxide, octadecanoid pathway
Faculty/Department:	Faculty of Health and Life Sciences > Department of Applied Sciences ~Pre-2010 Faculty Structure > Health and Life Sciences > School of Life Sciences ~Pre-2012 Faculty Structure > Faculty of Health and Life Sciences > Department of Applied Sciences
ID Code:	7089
Deposited By:	H. Hammond
Deposited On:	22 Jul 2010 07:56
Last Modified:	25 Feb 2013 15:21