Catherine E. Garner
Identification of Campylobacter infection in chickens from volatile faecal emissions
Garner, Catherine E.; Smith, Stephen; Elviss, Nicola C.; Humphrey, Tom J.; White, Paul; Ratcliffe, Norman M.; Probert, Christopher S.
Authors
Stephen Smith
Nicola C. Elviss
Tom J. Humphrey
Paul White Paul.White@uwe.ac.uk
Professor in Applied Statistics
Norman Ratcliffe Norman.Ratcliffe@uwe.ac.uk
Professor in Materials & Sensors Science
Christopher S. Probert
Abstract
Volatile organic compounds from chicken faeces were investigated as biomarkers for Campylobacter infection. Campylobacter are major poultry-borne zoonotic pathogens, colonizing the avian intestinal tract. Chicken faeces are the principal source of contamination of carcasses. Fresh faeces were collected on farm sites, and Campylobacter status established microbiologically. Volatile organic compounds were pre-concentrated from the headspace above 71 separate faecal samples using solid-phase microextraction and separated and identified by gas chromatography/mass spectrometry. A Campylobacter-specific profile was identified using six of the extracted volatile organic compounds. The model developed reliably identified the presence or absence of Campylobacter in >95% of chickens. The volatile biomarker identification approach for assessing avian infection is a novel approach to enhancing biosecurity in the poultry industry and should reduce the risk of disease transmission to humans.
Citation
Garner, C. E., Smith, S., Elviss, N. C., Humphrey, T. J., White, P., Ratcliffe, N. M., & Probert, C. S. (2008). Identification of Campylobacter infection in chickens from volatile faecal emissions. Biomarkers, 13(4), 413-421. https://doi.org/10.1080/13547500801966443
Journal Article Type | Article |
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Publication Date | Jun 1, 2008 |
Journal | Biomarkers |
Print ISSN | 1354-750X |
Electronic ISSN | 1366-5804 |
Publisher | Taylor & Francis |
Peer Reviewed | Peer Reviewed |
Volume | 13 |
Issue | 4 |
Pages | 413-421 |
DOI | https://doi.org/10.1080/13547500801966443 |
Keywords | Campylobacterinfection, chickens |
Public URL | https://uwe-repository.worktribe.com/output/1018680 |
Publisher URL | http://dx.doi.org/10.1080/13547500801966443 |