G. W. Davison
Molecular detection of exercise-induced free radicals following ascorbate prophylaxis in type 1 diabetes mellitus: A randomised controlled trial
Davison, G. W.; Ashton, T.; George, L.; Young, I. S.; McEneny, J.; Davies, B.; Jackson, Simon K.; Peters, J. R.; Bailey, D. M.
Authors
T. Ashton
L. George
I. S. Young
J. McEneny
B. Davies
Simon K. Jackson
J. R. Peters
D. M. Bailey
Abstract
Aims/hypothesis: Patients with type 1 diabetes mellitus are more susceptible than healthy individuals to exercise-induced oxidative stress and vascular endothelial dysfunction, which has important implications for the progression of disease. Thus, in the present study, we designed a randomised double-blind, placebo-controlled trial to test the original hypothesis that oral prophylaxis with vitamin C attenuates rest and exercise-induced free radical-mediated lipid peroxidation in type 1 diabetes mellitus. Methods: All data were collected from hospitalised diabetic patients. The electron paramagnetic resonance spectroscopic detection of spin-trapped α-phenyl-tert-butylnitrone (PBN) adducts was combined with the use of supporting markers of lipid peroxidation and non-enzymatic antioxidants to assess exercise-induced oxidative stress in male patients with type 1 diabetes (HbA1c 7.9±1%, n=12) and healthy controls (HbA1c 4.6±0.5%, n=14). Following participant randomisation using numbers in a sealed envelope, venous blood samples were obtained at rest, after a maximal exercise challenge and before and 2 h after oral ingestion of 1 g ascorbate or placebo. Participants and lead investigators were blinded to the administration of either placebo or ascorbate treatments. Primary outcome was the difference in changes in free radicals following ascorbate ingestion. Resuts: Six diabetic patients and seven healthy control participants were randomised to each of the placebo and ascorbate groups. Diabetic patients (n=12) exhibited an elevated concentration of PBN adducts (p
Citation
Davison, G. W., Ashton, T., George, L., Young, I. S., McEneny, J., Davies, B., …Bailey, D. M. (2008). Molecular detection of exercise-induced free radicals following ascorbate prophylaxis in type 1 diabetes mellitus: A randomised controlled trial. Diabetologia, 51(11), 2049-2059. https://doi.org/10.1007/s00125-008-1101-1
Journal Article Type | Article |
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Publication Date | Nov 1, 2008 |
Journal | Diabetologia |
Print ISSN | 0012-186X |
Publisher | Springer Verlag |
Peer Reviewed | Peer Reviewed |
Volume | 51 |
Issue | 11 |
Pages | 2049-2059 |
DOI | https://doi.org/10.1007/s00125-008-1101-1 |
Public URL | https://uwe-repository.worktribe.com/output/1007699 |
Publisher URL | http://dx.doi.org/10.1007/s00125-008-1101-1 |