Skip to main content

Research Repository

Advanced Search

Molecular detection of exercise-induced free radicals following ascorbate prophylaxis in type 1 diabetes mellitus: A randomised controlled trial

Davison, G. W.; Ashton, T.; George, L.; Young, I. S.; McEneny, J.; Davies, B.; Jackson, Simon K.; Peters, J. R.; Bailey, D. M.

Authors

G. W. Davison

T. Ashton

L. George

I. S. Young

J. McEneny

B. Davies

Simon K. Jackson

J. R. Peters

D. M. Bailey



Abstract

Aims/hypothesis: Patients with type 1 diabetes mellitus are more susceptible than healthy individuals to exercise-induced oxidative stress and vascular endothelial dysfunction, which has important implications for the progression of disease. Thus, in the present study, we designed a randomised double-blind, placebo-controlled trial to test the original hypothesis that oral prophylaxis with vitamin C attenuates rest and exercise-induced free radical-mediated lipid peroxidation in type 1 diabetes mellitus. Methods: All data were collected from hospitalised diabetic patients. The electron paramagnetic resonance spectroscopic detection of spin-trapped α-phenyl-tert-butylnitrone (PBN) adducts was combined with the use of supporting markers of lipid peroxidation and non-enzymatic antioxidants to assess exercise-induced oxidative stress in male patients with type 1 diabetes (HbA1c 7.9±1%, n=12) and healthy controls (HbA1c 4.6±0.5%, n=14). Following participant randomisation using numbers in a sealed envelope, venous blood samples were obtained at rest, after a maximal exercise challenge and before and 2 h after oral ingestion of 1 g ascorbate or placebo. Participants and lead investigators were blinded to the administration of either placebo or ascorbate treatments. Primary outcome was the difference in changes in free radicals following ascorbate ingestion. Resuts: Six diabetic patients and seven healthy control participants were randomised to each of the placebo and ascorbate groups. Diabetic patients (n=12) exhibited an elevated concentration of PBN adducts (p

Citation

Davison, G. W., Ashton, T., George, L., Young, I. S., McEneny, J., Davies, B., …Bailey, D. M. (2008). Molecular detection of exercise-induced free radicals following ascorbate prophylaxis in type 1 diabetes mellitus: A randomised controlled trial. Diabetologia, 51(11), 2049-2059. https://doi.org/10.1007/s00125-008-1101-1

Journal Article Type Article
Publication Date Nov 1, 2008
Journal Diabetologia
Print ISSN 0012-186X
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 51
Issue 11
Pages 2049-2059
DOI https://doi.org/10.1007/s00125-008-1101-1
Public URL https://uwe-repository.worktribe.com/output/1007699
Publisher URL http://dx.doi.org/10.1007/s00125-008-1101-1


Downloadable Citations