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DPPC regulates COX-2 expression in monocytes via phosphorylation of CREB

Morris, R. H.K.; Jones, K. P.; Ahluwalia, M. K.; Tonks, Amanda J.; Thomas, A.W; Jackson, Simon K.

Authors

R. H.K. Morris

K. P. Jones

M. K. Ahluwalia

Amanda J. Tonks

A.W Thomas

Simon K. Jackson



Abstract

The major phospholipid in pulmonary surfactant dipalmitoyl phosphatidylcholine (DPPC) has been shown to modulate inflammatory responses. Using human monocytes, this study demonstrates that DPPC significantly increased PGE2 (P < 0.05) production by 2.5-fold when compared to untreated monocyte controls. Mechanistically, this effect was concomitant with an increase in COX-2 expression which was abrogated in the presence of a COX-2 inhibitor. The regulation of COX-2 expression was independent of NF-κB activity. Further, DPPC increased the phosphorylation of the cyclic AMP response element binding protein (CREB; an important nuclear transcription factor important in regulating COX-2 expression). In addition, we also show that changing the fatty acid groups of PC (e.g. using l-α-phosphatidylcholine β-arachidonoyl-γ-palmitoyl (PAPC)) has a profound effect on the regulation of COX-2 expression and CREB activation. This study provides new evidence for the anti-inflammatory activity of DPPC and that this activity is at least in part mediated via CREB activation of COX-2. © 2008 Elsevier Inc. All rights reserved.

Citation

Ahluwalia, M. K., Jones, K. P., Morris, R. H., Tonks, A. J., Thomas, A., & Jackson, S. K. (2008). DPPC regulates COX-2 expression in monocytes via phosphorylation of CREB. Biochemical and Biophysical Research Communications, 370(1), 174-178. https://doi.org/10.1016/j.bbrc.2008.03.052

Journal Article Type Article
Publication Date May 23, 2008
Journal Biochemical and Biophysical Research Communications
Print ISSN 0006-291X
Electronic ISSN 1090-2104
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 370
Issue 1
Pages 174-178
DOI https://doi.org/10.1016/j.bbrc.2008.03.052
Keywords pulmonary surfactant, inflammation, DPPC, COX-2, CREB
Public URL https://uwe-repository.worktribe.com/output/1012417
Publisher URL http://dx.doi.org/10.1016/j.bbrc.2008.03.052




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