R. H.K. Morris
DPPC regulates COX-2 expression in monocytes via phosphorylation of CREB
Morris, R. H.K.; Jones, K. P.; Ahluwalia, M. K.; Tonks, Amanda J.; Thomas, A.W; Jackson, Simon K.
Authors
K. P. Jones
M. K. Ahluwalia
Amanda J. Tonks
A.W Thomas
Simon K. Jackson
Abstract
The major phospholipid in pulmonary surfactant dipalmitoyl phosphatidylcholine (DPPC) has been shown to modulate inflammatory responses. Using human monocytes, this study demonstrates that DPPC significantly increased PGE2 (P < 0.05) production by 2.5-fold when compared to untreated monocyte controls. Mechanistically, this effect was concomitant with an increase in COX-2 expression which was abrogated in the presence of a COX-2 inhibitor. The regulation of COX-2 expression was independent of NF-κB activity. Further, DPPC increased the phosphorylation of the cyclic AMP response element binding protein (CREB; an important nuclear transcription factor important in regulating COX-2 expression). In addition, we also show that changing the fatty acid groups of PC (e.g. using l-α-phosphatidylcholine β-arachidonoyl-γ-palmitoyl (PAPC)) has a profound effect on the regulation of COX-2 expression and CREB activation. This study provides new evidence for the anti-inflammatory activity of DPPC and that this activity is at least in part mediated via CREB activation of COX-2. © 2008 Elsevier Inc. All rights reserved.
Citation
Ahluwalia, M. K., Jones, K. P., Morris, R. H., Tonks, A. J., Thomas, A., & Jackson, S. K. (2008). DPPC regulates COX-2 expression in monocytes via phosphorylation of CREB. Biochemical and Biophysical Research Communications, 370(1), 174-178. https://doi.org/10.1016/j.bbrc.2008.03.052
Journal Article Type | Article |
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Publication Date | May 23, 2008 |
Journal | Biochemical and Biophysical Research Communications |
Print ISSN | 0006-291X |
Electronic ISSN | 1090-2104 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 370 |
Issue | 1 |
Pages | 174-178 |
DOI | https://doi.org/10.1016/j.bbrc.2008.03.052 |
Keywords | pulmonary surfactant, inflammation, DPPC, COX-2, CREB |
Public URL | https://uwe-repository.worktribe.com/output/1012417 |
Publisher URL | http://dx.doi.org/10.1016/j.bbrc.2008.03.052 |