W. Terence Coakley
Long-term viability and proliferation of alginate-encapsulated 3-D HepG2 aggregates formed in an ultrasound trap
Coakley, W. Terence; Hayes, Adrian; Bazou, D.; Jackson, Simon K.
Authors
Adrian Hayes
D. Bazou
Simon K. Jackson
Abstract
We report proof of principle here of a gel encapsulation technique that departs from the minimum surface area to volume restriction of spherical microcapsules and allows gelation of preformed high-density (≥2 × 104 cells/aggregate) 3-D HepG2 cell aggregates. The process involves forming a discoid 3-D cell aggregate in an ultrasound standing wave trap (USWT), which is subsequently recovered and encapsulated in alginate/CaCl2 hydrogel. The size of the ultrasound-formed aggregates was dependent upon the initial cell concentration, and was in the range of 0.4-2.6 mm in diameter (for cell concentrations ranging between 104 and 5 × 106/ml). At low cell concentrations (≤5 × 105/ml), aggregates were 2-D, while at concentrations of ≥106/ml, 3-D aggregates were generated. Cells in non- and encapsulated 3-D HepG2 aggregates remained 70-80% viable over 10 days in culture. The proliferative activity of the aggregates resulted in the doubling of the aggregate cell number and a subsequent increase in the aggregate thickness, while albumin secretion levels in encapsulated aggregates was 4.5 times higher compared to non-encapsulated, control aggregates. The results reported here suggest that the ultrasound trap can provide an alternative, novel approach of hydrogel cell encapsulation and thus rapidly (within 5 min) produce in vitro models for hepatocyte functional studies (for example, toxicity studies particularly if primary hepatocytes are used) in a tissue-mimetic manner. © 2008 Elsevier Ltd. All rights reserved.
Citation
Bazou, D., Coakley, W. T., Hayes, A., & Jackson, S. K. (2008). Long-term viability and proliferation of alginate-encapsulated 3-D HepG2 aggregates formed in an ultrasound trap. Toxicology in Vitro, 22(5), 1321-1331. https://doi.org/10.1016/j.tiv.2008.03.014
Journal Article Type | Article |
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Publication Date | Aug 1, 2008 |
Journal | Toxicology in Vitro |
Print ISSN | 0887-2333 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 22 |
Issue | 5 |
Pages | 1321-1331 |
DOI | https://doi.org/10.1016/j.tiv.2008.03.014 |
Keywords | alginate, ultrasound trap, F-actin, 2-/3-D cell aggregate, viability, proliferation |
Public URL | https://uwe-repository.worktribe.com/output/1010291 |
Publisher URL | http://dx.doi.org/10.1016/j.tiv.2008.03.014 |