Radhika Desikan
Tools to investigate ROS sensitive signalling proteins
Desikan, Radhika; Neill, Steven; Slinn, Jenna; Hancock, John T.
Abstract
The thiols groups of cysteine residues on proteins are attractive oxidative targets for modification by reactive oxygen species, such as hydrogen peroxide (H2O2). Such modification can lead to important cellular signaling processes that ultimately result in modification of physiology of the organism. To identify such proteins that are amenable to oxidative modification, different methods can be used. Here, two such approaches are described: one being the use of fluorescent thiol derivatives, and the second being the use of genetic mutants that are mutated in thiol residues. Using the model plant Arabidopsis thaliana, cell cultures, and whole plants, we describe these tools to help the reader understand the function of such thiol modification on plant responses. © 2008 Humana Press, a part of Springer Science+Business Media, LLC.
Citation
Desikan, R., Neill, S., Slinn, J., & Hancock, J. T. (2008). Tools to investigate ROS sensitive signalling proteins. Methods in Molecular Biology, 476, 87-99. https://doi.org/10.1007/978-1-59745-129-1_7
Journal Article Type | Article |
---|---|
Publication Date | Jan 1, 2008 |
Journal | Methods in Molecular Biology |
Print ISSN | 1064-3745 |
Publisher | Humana Press |
Peer Reviewed | Not Peer Reviewed |
Volume | 476 |
Pages | 87-99 |
Series Title | Methods in Molecular Biology |
DOI | https://doi.org/10.1007/978-1-59745-129-1_7 |
Keywords | molecular biology, redox-mediated signal transduction, ROS sensitive signalling proteins |
Public URL | https://uwe-repository.worktribe.com/output/1018099 |
Publisher URL | http://www.springerprotocols.com/BookToc/doi/10.1007/978-1-59745-129-1 |
You might also like
An interplay of gases: oxygen and hydrogen in biological systems
(2024)
Journal Article
Reactive oxygen, nitrogen, and sulfur species cellular crosstalk
(2023)
Book Chapter
H2S priming and plant abiotic stress tolerance
(2023)
Book Chapter
Molecular hydrogen as a beneficial postharvest treatment
(2023)
Book Chapter