The challenges of the analysis of basic compounds by high performance liquid chromatography: Some possible approaches for improved separations

McCalley, D. V. (2010) The challenges of the analysis of basic compounds by high performance liquid chromatography: Some possible approaches for improved separations. Journal of Chromatography A, 1217 (6). pp. 858-880. ISSN 0021-9673

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Publisher's URL: http://dx.doi.org/10.1016/j.chroma.2009.11.068

Abstract

This review considers some of the difficulties encountered with the analysis of ionised bases using reversed-phase chromatography, such as detrimental interaction with column silanol groups, and overloading which both lead to poor peak shapes. Methods of overcoming these problems in reversed-phase (RP) separations, by judicious selection of the column and mobile phase conditions, are discussed. Hydrophilic interaction chromatography is considered as an alternative method for the separation of some basic compounds.

Item Type:Article
Uncontrolled Keywords:pharmaceuticals, biomedically significant compounds, HPLC basic compounds, HPLC, stationary phases, reversed-phase HILIC
Faculty/Department:Faculty of Health and Applied Sciences > Department of Biological, Biomedical and Analytical Sciences
ID Code:8284
Deposited By: Professor D. Mccalley
Deposited On:09 Jul 2010 10:10
Last Modified:12 Aug 2013 08:00

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