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Investigation of the effect of pressure on retention of small molecules using reversed-phase ultra-high-pressure liquid chromatography

Fallas, Morgane M.; Neue, Uwe D.; Hadley, Mark R.; McCalley, David V.

Authors

Morgane M. Fallas

Uwe D. Neue

Mark R. Hadley



Abstract

The effect of inlet pressure on the retention of a series of low molecular weight acids, bases and neutrals, was investigated at constant temperature in reversed-phase liquid chromatography using a commercial ultra-high-pressure system (Waters UPLC instrument). For neutral compounds, relatively small increases in retention factor of up to ∼12% for a pressure increase of 500 bar were noted; the largest values were obtained for polar solutes, or solutes of higher molecular weight. Ionisable acids and bases gave much larger increases in retention with pressure, in some cases as high as 50% for a pressure increase of 500 bar. Thus, such compounds could show increases in retention factor approaching 100% over the pressure range available in the commercial UPLC instrument. Due to these differential increases, significant selectivity effects can be obtained for mixtures of different types of solute merely by changing the pressure. © 2008 Elsevier B.V. All rights reserved.

Citation

Fallas, M. M., Neue, U. D., Hadley, M. R., & McCalley, D. V. (2008). Investigation of the effect of pressure on retention of small molecules using reversed-phase ultra-high-pressure liquid chromatography. Journal of Chromatography A, 1209(1-2), 195-205. https://doi.org/10.1016/j.chroma.2008.09.021

Journal Article Type Article
Publication Date Oct 31, 2008
Journal Journal of Chromatography A
Print ISSN 0021-9673
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 1209
Issue 1-2
Pages 195-205
DOI https://doi.org/10.1016/j.chroma.2008.09.021
Keywords retention factors, variation with pressure, ultra-high-pressure LC, reversed-phase
Public URL https://uwe-repository.worktribe.com/output/1008069
Publisher URL http://dx.doi.org/10.1016/j.chroma.2008.09.021