Investigation of the effect of pressure on retention of small molecules using reversed-phase ultra-high-pressure liquid chromatography

Fallas, M. M., Neue, U. D., Hadley, M. R. and McCalley, D. (2008) Investigation of the effect of pressure on retention of small molecules using reversed-phase ultra-high-pressure liquid chromatography. Journal of Chromatography A, 1209 (1-2). pp. 195-205. ISSN 0021-9673

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Publisher's URL: http://dx.doi.org/10.1016/j.chroma.2008.09.021

Abstract

The effect of inlet pressure on the retention of a series of low molecular weight acids, bases and neutrals, was investigated at constant temperature in reversed-phase liquid chromatography using a commercial ultra-high-pressure system (Waters UPLC instrument). For neutral compounds, relatively small increases in retention factor of up to 12% for a pressure increase of 500 bar were noted; the largest values were obtained for polar solutes, or solutes of higher molecular weight. Ionisable acids and bases gave much larger increases in retention with pressure, in some cases as high as 50% for a pressure increase of 500 bar. Thus, such compounds could show increases in retention factor approaching 100% over the pressure range available in the commercial UPLC instrument. Due to these differential increases, significant selectivity effects can be obtained for mixtures of different types of solute merely by changing the pressure.

Item Type:Article
Uncontrolled Keywords:retention factors, variation with pressure, ultra-high-pressure LC, reversed-phase
Faculty/Department:Faculty of Health and Applied Sciences > Department of Biological, Biomedical and Analytical Sciences
ID Code:8299
Deposited By: Professor D. McCalley
Deposited On:12 Jul 2010 09:00
Last Modified:12 Aug 2013 08:00

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