DNA microarray screening of differential gene expression in bone marrow samples from AML, non-AML patients and AML cell lines
Court, E. , Smith, M. A. , Avent, N. D. , Hancock, J. T. , Morgan, L. , Gray, A. and Smith, J. G. (2003) DNA microarray screening of differential gene expression in bone marrow samples from AML, non-AML patients and AML cell lines. Leukemia Research, 28 (7). pp. 743-753. ISSN 0145-2126
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Publisher's URL: http://dx.doi.org/10.1016/j.leukres.2003.11.011
This study used cDNA microarray technology to compare gene expression profiles in acute myeloblastic leukaemia (AML) with cDNA dot-blot and real time PCR analysis of cDNA transcripts to confirm array data. Patient AML marrow samples and AML cell lines were compared with normal/non-AML samples. Screening revealed five particular genes to be significantly differentially expressed across the sample groups. The migration-inhibitory factor-related-proteins 8 and 14 (MRP-8 and MRP-14) genes, the products of which inhibit cell migration and differentiation were the most highly expressed in non-malignant cells. The high-mobility-group-protein gene (HMG-1) was up regulated in leukaemic samples and cell lines, which may be associated with aggressive disease. Also upregulated in malignant samples were genes encoding c-myc and glutathione−S−transferase pi (GSTP), the latter implicated in chemotherapy resistance. Faulty expression of such genes may contribute to the pathogenesis of AML and resistance to treatment.
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