Fabrication of microband glucose biosensors using a screen-printing water-based carbon ink and their application in serum analysis
Pemberton, R., Pittson, R., Biddle, N. and Hart, J. P. (2009) Fabrication of microband glucose biosensors using a screen-printing water-based carbon ink and their application in serum analysis. Biosensors and Bioelectronics, 24 (5). pp. 1246-1252. ISSN 0956-5663 Available from: http://eprints.uwe.ac.uk/8331
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Publisher's URL: http://dx.doi.org/10.1016/j.bios.2008.07.035
Microband glucose biosensors were fabricated by screen-printing a water-based carbon ink formulation containing cobalt phthalocyanine redox mediator and glucose oxidase (GOD) enzyme, then insulating and sectioning through the thick (20 μm) film to expose a 3 mm-long working electrode edge. The performance of these biosensors for glucose analysis was investigated at 25 °C. Voltammetry in glucose-containing buffer solutions established that an operating potential of +0.4 V vs. Ag/AgCl was suitable for analysis under both stirring and quiescent conditions. The influence of pH on biosensor performance was established and an operational pH of 8.0 was selected. Steady-state responses were obtained under quiescent conditions, suggesting a mixed mechanism predominated by radial diffusion, indicative of microelectrode behaviour. Calibration studies obtained with these biosensors showed steady-state currents that were linearly dependent on glucose concentration from the limit of detection (0.27 mM) up to 2.0 mM, with a precision for replicate biosensors of 6.2–10.7%. When applied to the determination of glucose in human serum, the concentration compared favourably to that determined by a spectroscopic method. These results have demonstrated a simple means of fabricating biosensors for glucose measurement and determination in situations where low-current real-time monitoring under quiescent conditions would be desirable.